Annual Courses

GC (x GC) –MS(/MS) in the 21st Century

October 2nd to 4th at the New Brighten Community Center 

Register Here

ROBERT J KOBELSKI

At the turn of the 21st century, or the third millennium if you prefer, the marriage of GC-MS had become mature, staid, boring. No more the heady days of the introduction of narrow bore fused silica open tubular columns by HP’s Avondale Division, no more market explosions like the introduction of the benchtop quadrupole mass spectrometer, mass selective detector, by HP’s Scientific Instruments Division. Just a series of minor platform changes, small enhancements in sensitivity through remedying design defects, adding some bells a whistles like large volume injection but really just a nice technology that that many were comfortable with that dominated any number of applications but didn’t have much of a future.

Then things started to change in the marriage; hormone therapy and Viagra™ were introduced. Technologies developed or refined for LC-MS applications such as tandem MS and HRAM MS found their way into the GC-MS market breathing new life into the staid single quadrupole platform. While MS was learning how to MS/MS GC was not standing still; just as large volume injection had overcome one of the limitations of narrow-bore columns the development of GC x GC had taken an already highly efficient separation technique and made it better and this change paired perfecting with benchtop Time-of-Flight mass spectrometers (with or without HRAM). Date nights had become exciting once again.

The objective of this course is to provide an understanding of: the fundamental chemistries involved in mass spectrometry including ionization techniques, the principles of quadrupole and time-of-flight mass analysis both as single stage and tandem systems, the science at the core of gas chromatographic resolution and the integration of sample introduction into that science, the use of the mass spectrometers for qualitative analysis including both data acquisition and analysis, and the use of the mass spectrometer as a detector for the gas chromatograph for highly specific and sensitive quantitative analysis.

Course Outline

  1. Chemistry of Mass Spectrometry
    • Atomic and molecular mass
    • Molecular fragmentation
    • Stable isotope information
  2. Ionization
    • Electron ionization
    • Chemical ionization
      • Positive Chemical Ionization (PCI)
      • Negative Chemical Ionization (NCI)
  3. Mass Analysis
    • Time-of-flight (TOF))
    • Quadrupole
    • Tandem
      • Triple quad
      • Q-TOF
  4. Resolution in Gas Chromatography
    • Retention time
    • Peak width
    • Efficiency
    • Capacity factor
    • Selectivity
  5. GC Sample Introduction
    • Split
    • Splitless
    • Large volume injection (LVI or PTV)
  6. Qualitative Analysis Data Acquisition
    • Quadrupole acquisition process
    • Quadrupole acquisition parameters
    • TOF acquisition process
    • TOF acquisition parameters
    • Tandem systems
      • Product Scan
      • Precursor scan
      • Neutral loss
  7. Qualitative Analysis Data Analysis
    • Creating a spectrum for analysis
    • Library searching (NIST)
    • Basic interpretation tools
  8. Quantitative Analysis Data Acquisition
    • Quadrupole acquisition process
    • Quadrupole acquisition parameters
    • Tandem systems
      • SRM
  9. Quantitative analysis Data Analysis
    • Extracting signals & specificity
      • Retention time
      • Quantitative/qualitative signal ratios
      • LOD/LOD
    • Quantitative methods
      • Calibration
      • External Standard
      • Standard Addition
      • Internal Standard