Symposium Short Courses

“Analytical Sampling and Sample Preparation for Chromatography”

by Dr. Douglas Raynie

 

Course Description

This short course is designed to provide participants with an in-depth understanding of the role of sampling and sample preparation in chromatography.  Upon successful completion of the course, the participant will have an understanding of sampling consideration and approaches, and sample preparation strategies.  The course will begin by discussing the role of sampling, sample types, sample size, sample storage and handling, etc.  Next, general laboratory skills that are often overlooked, but which greatly impact the overall analysis, will be reviewed.  The heart of the course will be the presentation of traditional and newly developed chemical extraction methods for sample preparation.  Finally, post-extraction sample treatment will be addressed. Throughout the course practical and theoretical aspects of the outlined topics and application case studies will be presented.

 

Course Outline

  • Introduction
  • Statistical Considerations
  • Sampling
  • General Laboratory Practices
  • General Extraction Considerations
  • Extraction From Liquid Samples
  • Extraction From Solid Samples
  • Chemical Extractions – Special Cases
  • Extraction Of Volatile Analytes
  • Post-Extraction Sample Handling

 

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DOUGLAS E. RAYNIE

 

Dr. Doug Raynie is Department Head and Associate Professor in the Department of Chemistry and Biochemistry at South Dakota State University. Prior to joining SDSU, he was employed for eleven years as a Senior Scientist at Procter and Gamble’s Corporate Research Division. He earned his Ph.D. at Brigham Young University under the direction of Dr. Milton L. Lee. His undergraduate degree is from Augustana (South Dakota) College, with majors in chemistry and biology.

 

Dr. Raynie’s broad research interests are in the field of sustainability and green chemistry. His two major areas of research are bioprocessing using supercritical fluids and analytical separations. Current research is centered on the development of deep eutectic solvents for novel applications and applications of high-resolution chromatography to food science applications. Analytical separations research includes high-resolution chromatography (high-temperature LC and SFC), chromatographic sample preparation (ASE, SFE, SPME, and SPE), chromatography theory, green analytical chemistry, and problem-based learning in analytical chemistry. He serves as Sample Preparation Perspectives columnist for LC/GC magazine.

 

“Comprehensive GC & GC-MS Troubleshooting”

by Rick Rossiter

 Every practicing chromatographer is going to meet trouble sometime.

 

This 1 ½ day course will help you:

 

Recognize when something is wrong

Brainstorm possible causes for the problem

Use information you may already have to narrow down these possibilities

Suggest simple tests you might do to confirm the actual cause

Know what you can do to fix the problem

Know when to call in reinforcements

 

Course outline

Philosophies of troubleshooting Tools

Proper function of, and problematic conditions of:

Flows, Injectors, Autosamplers, Columns,

Detectors (primarily FID and MS, but others as requested), Data handling

When is it not an instrument problem?

Review, practice and discussion

 

We do not guarantee to solve your problems for you, but you will leave the course better equipped to do so for yourself!

 

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Rick Rossiter, MA [Oxon]

Rick holds a Master’s degree in Chemistry from the University of Oxford in the UK.

He taught high school Chemistry in Colchester, England for ten years before moving to Minnesota in 1982, following his marriage to a native of Dinkytown, Minneapolis.

 

Here he was fortunate enough to obtain a position in the 3M Corporate Research Analytical Laboratory, which provides global analytical leadership for that diverse company.  For over thirty years he performed analyses and solved problems using GC, LC, SFC and CE, coupled to a wide variety of detectors including mass spectrometry and atomic emission.

 

Rick has been a co-instructor for the MCF Beginning GC course for several years, and a regular presenter at the MCF Spring Symposium.

 

He retired in 2016, but continues to volunteer as a member of the 3M Visiting Wizards, who provide science experiences for elementary students [and sometimes for their parents also].

 

“Advanced HPLC and UHPLC 

Method Development Using Quality by Design (QbD)”

 by John Dolan

The central tenant of Quality by Design (ICH Q8) is that quality cannot be tested into a product – instead it must be designed into the product. When the product is an HPLC or UHPLC method, QbD strategies can guide the development process to result in a standardized method development process, more easily validated methods, and methods that are easier to use and adjust in routine applications.

 

This 1.5- day class is designed for laboratory workers involved in HPLC method development as well as those who must transfer of existing methods into a routine laboratory. The class focuses on separation fundamentals and applies them in a time-proven strategy that applies QbD principles to developing robust HPLC methods quickly. The use of UHPLC and/or new particle technologies can enhance comprehensive method development using QbD. Based on the instructor’s extensive experience in a laboratory supporting pharmaceutical and bioanalytical methods, the attendees should gain practical skills to develop realistic HPLC methods in a short time. The techniques can be used as a stand-alone strategy or added to existing development procedures to help streamline the process. The course content assumes a basic understanding of HPLC, but not necessarily experience in method development. Students will be introduced to HPLC method development software to help find satisfactory separation conditions. Attendees will receive a comprehensive set of course notes, including copies of all visual materials presented.

 

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Dr. John Dolan

Dr. John Dolan is a Principal Trainer and consultant for LC Resources, Inc.. John received his Ph.D. from the University of California at Davis in 1976 and has more than 40 years of HPLC experience. After finishing graduate school, he did postdoctoral work at Northeastern University and then joined Technicon Instruments Corporation, where he worked for three years developing clinical HPLC technology. He moved to IBM Instruments, where he was involved in design and support of LC, IR, and UV products. As a columnist for LC/GC magazine, he has written over 340 installments of the “LC Troubleshooting” monthly column since 1983. In 1984, John and Lloyd Snyder founded LC Resources, which offered support to the separations community via teaching, software, consulting, and laboratory services. In 2002, LC Resources sold its DryLab software products to Rheodyne, the laboratory to Bioanalytical Systems, and retained the training business. After acting as General Manager of the BASi Northwest Laboratory for three years, John now spends full time teaching and consulting. He has co-authored three books and more than 125 scientific papers on LC theory, instrumentation, and applications as well as a book on troubleshooting LC instruments and methods. John is the 2002 recipient of the MCF Palmer Award and the 2007 Dal Nogare from the Chromatography Forum of Delaware Valley.